Fluorescent Cell Proliferation Assay

Catalog number INDBLU100-3P

Discontinued

Price for INDBLU100-3P

Size / count 500 Tests

This variant is discontinued and cannot be added to your cart.

About this product

An oxidation/reduction-based reagent quantitatively measures the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When reduced by viable cells, it fluoresces red. Read in a fluorescent plate reader.

Specifications

Catalog number: INDBLU100-3P
Size / count: 500 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Cell Type: Jurkat
Type: Metabolic Assays
Detection Method: Fluorescence plate reader
Wavelength: 530 nm and Emission = 590 nm /absorbance at 570nm
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_IndoBlu_Protocol.pdf?v=1693430859
Components: Part # 4024: IndoBluTM Reagent (2-8C), 96-Well Clear Bottomed Black Plate for Fluorescent Readout x 5
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: -20°C
Target Description: Measurement of cell proliferation and viability is frequently used in clinical and experimental immunology as means of assessing cell activation in response to diseases, infections and environmental stimulations. This assay can be used for the measurement of cell proliferation in response to antigens, cytokines, growth factors, and mitogens. It can also be used for the analysis of cytotoxic effects of anticancer drugs, drug resistance, cytotoxic pharmaceutical compounds, and other toxic agents. This kit uses an oxidation/reduction-based reagent that functions as a cell viability indicator by quantitatively measuring the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When added to cells, the reagent is modified by the reducing environment of the viable cell, it turns red in color and becomes highly fluorescent. This change can be detected using fluorescence or absorbance measurements. The reagent is detected at Excitation = 530 nm and Emission = 590 nm. Absorbance is measured at 570 nm. Compared to the traditional cell proliferation assay, our novel in-cell-culture method is simple, fast and sensitive. The entire assay can be performed in a 96-Well microtiter plate in 30 minutes and the best results are achieved in 3 hours.

Frequently Asked Questions

What is the catalog number for Fluorescent Cell Proliferation Assay?

The catalog number for Fluorescent Cell Proliferation Assay is INDBLU100-3P.

Is Fluorescent Cell Proliferation Assay in stock?

Fluorescent Cell Proliferation Assay is currently made to order / not held in immediate stock. Contact us for lead time.

What is Fluorescent Cell Proliferation Assay used for?

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.