Fluorescent Cell Proliferation Assay

Catalog number INDBLU100-4

Discontinued

Price for INDBLU100-4

Size / count 1000 Tests

This variant is discontinued and cannot be added to your cart.

About this product

An oxidation/reduction-based reagent quantitatively measures the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When reduced by viable cells, it fluoresces red. Read in a fluorescent plate reader.

Specifications

Catalog number: INDBLU100-4
Size / count: 1000 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Cell Type: Jurkat
Type: Metabolic Assays
Detection Method: Fluorescence plate reader
Wavelength: 530 nm and Emission = 590 nm /absorbance at 570nm
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_IndoBlu_Protocol.pdf?v=1693430859
Components: Part # 4024: IndoBluTM Reagent x 2 (2-8C), Optional: 96-Well Clear Bottomed Black Plate for Fluorescent Readout
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: -20°C
Target Description: Measurement of cell proliferation and viability is frequently used in clinical and experimental immunology as means of assessing cell activation in response to diseases, infections and environmental stimulations. This assay can be used for the measurement of cell proliferation in response to antigens, cytokines, growth factors, and mitogens. It can also be used for the analysis of cytotoxic effects of anticancer drugs, drug resistance, cytotoxic pharmaceutical compounds, and other toxic agents. This kit uses an oxidation/reduction-based reagent that functions as a cell viability indicator by quantitatively measuring the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When added to cells, the reagent is modified by the reducing environment of the viable cell, it turns red in color and becomes highly fluorescent. This change can be detected using fluorescence or absorbance measurements. The reagent is detected at Excitation = 530 nm and Emission = 590 nm. Absorbance is measured at 570 nm. Compared to the traditional cell proliferation assay, our novel in-cell-culture method is simple, fast and sensitive. The entire assay can be performed in a 96-Well microtiter plate in 30 minutes and the best results are achieved in 3 hours.

Frequently Asked Questions

What is the catalog number for Fluorescent Cell Proliferation Assay?

The catalog number for Fluorescent Cell Proliferation Assay is INDBLU100-4.

Is Fluorescent Cell Proliferation Assay in stock?

Fluorescent Cell Proliferation Assay is currently made to order / not held in immediate stock. Contact us for lead time.

What is Fluorescent Cell Proliferation Assay used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.