Fluorescent Cell Proliferation Assay

About this product

An oxidation/reduction-based reagent quantitatively measures the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When reduced by viable cells, it fluoresces red. Read in a fluorescent plate reader.

Specifications

Catalog number

INDBLU100-4P

Size / count

1000 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Cell Type

Jurkat

Type

Metabolic Assays

Detection Method

Fluorescence plate reader

Wavelength

530 nm and Emission = 590 nm /absorbance at 570nm

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_IndoBlu_Protocol.pdf?v=1693430859

Components

Part # 4024: IndoBluTM Reagent x 2 (2-8C), 96-Well Clear Bottomed Black Plate for Fluorescent Readout x 10

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

-20°C

Target Description

Measurement of cell proliferation and viability is frequently used in clinical and experimental immunology as means of assessing cell activation in response to diseases, infections and environmental stimulations. This assay can be used for the measurement of cell proliferation in response to antigens, cytokines, growth factors, and mitogens. It can also be used for the analysis of cytotoxic effects of anticancer drugs, drug resistance, cytotoxic pharmaceutical compounds, and other toxic agents. This kit uses an oxidation/reduction-based reagent that functions as a cell viability indicator by quantitatively measuring the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When added to cells, the reagent is modified by the reducing environment of the viable cell, it turns red in color and becomes highly fluorescent. This change can be detected using fluorescence or absorbance measurements. The reagent is detected at Excitation = 530 nm and Emission = 590 nm. Absorbance is measured at 570 nm. Compared to the traditional cell proliferation assay, our novel in-cell-culture method is simple, fast and sensitive. The entire assay can be performed in a 96-Well microtiter plate in 30 minutes and the best results are achieved in 3 hours.