Fluorescent Semicarbazide-Sensitive Amine Oxidase Detection Kit

About this product

This kit utilizes a non-fluorescent detection reagent to measure H2O2 released from the conversion of benzylamine to benzaldehyde via Semicarbazide-Sensitive Amine Oxidase. H2O2 then oxidizes the detection reagent to produce a fluorescent product.

Specifications

Catalog number

SSAO100-3

Size / count

500 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Metabolic Assays

Detection Method

Fluorescence plate reader

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/SSAOProtocol.pdf?v=1693431069

Components

Part # 3019: 5X Reaction Buffer pH 7.4, 1 Bottle (2-8C), Part # 6005: Horseradish Peroxidase, 1 Vial (2-8C), Part # 4008: Detection Reagent, 1 Vial (-20C (Aliquot in Single Use Vials)), Part # 7001: SSAO Substrate Benzylamine, 1 Vial (-20C (Aliquot in Single Use Vials)), Part # 6006: SSAO Enzyme, 1 Vial (-20C (Aliquot in Single Use Vials)), Part # 7003: Pargyline: Monoamine Oxidase B Inhibitor(9-11), 1 Vial (-20C (Aliquot in Single Use Vials)), Part # 7004: Semicarbazide-Sensitive Amine Oxidase Inhibitor, 1 Vial (-20C (Aliquot in Single Use Vials))

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

Store contents as labeled

Target Description

Semicarbazide-sensitive amine oxidase (SSAO) is a common name for a widely distributed enzyme in nature. In man, this enzyme is present in the vascular system and circulates in plasma. SSAO differs from the monoamine oxidases A and B in substrate and inhibitor patterns. These enzymes have been widely studied and their tissue distribution, molecular properties, substrate specificities and inhibitor sensitivities are extensively reviewed. SSAO exists in two forms: tissue bound and soluble (plasma SSAO). Tissue bound SSAO activity is associated with blood vessels, mainly in smooth muscle layers, however it is also associated with spleen, placenta, bone marrow, kidney, sclera, retina, endothelial cells, adipocytes, chondrocytes and fibroblasts. It is expected and evidence suggests that plasma SSAO originates from the cleavage of membrane-bound form. The possible sources of plasma SSAO are still unclear, but it has been suggested that it may be derived from liver, retina, placenta and bone tissue. SSAO’s functional role has been suggested to be involved in: apoptosis, atherogenesis, cell adhesion, leukocyte trafficking, glucose transport and local production of hydrogen peroxide. Elevated levels of SSAO have been reported in congestive heart failure, diabetes mellitus, Alzheimer's disease and various other inflammatory diseases. Furthermore, by products of SSAO deamination, such as formaldehyde and methylglyoxal, have been proposed to be involved in pathogenesis of cancer, aging and atherosclerosis. The Fluorescent Semicarbazide-Sensitive Amine Oxidase Detection Kit utilizes a non-fluorescent detection reagent to measure H2O2 released from the conversion of Benzylamine to Benzaldehyde via SSAO. H2O2 then oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by peroxidase.