Recombinant Human CXCL1 Protein (Trx Tag)

Specifications

Catalog number

PDEH100660-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a) . CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.

Abbreviation

CXCL1

Synonyms

CXCL; SCYB; Secretory protein N; CINC; NAP; CXCL1; FSP; GRO1; GROa; MGSA; MGSA-a; NAP-3; SCYB1; GRO alpha; CINC-1; C-X-C Motif Chemokine 1; GRO-A; GRO-Alpha (1-73) ; Growth-Regulated Alpha Protein; GRO-α: MGSAα; Melanoma Growth Stimulatory Activity; NAP3; Neutrophil-Activating Protein 3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; GRO; alpha; alpha) ; chemokine (C-X-C motif) ligand 1; Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; C-X-C motif chemokine ligand 1; Cytokine-induced neutrophil chemoattractant 1; Fibroblast secretory protein; Gro 1; Gro A; GRO protein; Gro1 oncogene; GRO1 oncogene (melanoma growth stimulating activity; GRO1 oncogene (melanoma growth-stimulating activity) ; GRO-alpha (6-73) ; homolog of; KC chemokine; melanoma growth stimulatory activity alpha; member 1; MGSA alpha; mouse; N51; Scyb 1; Small inducible cytokine subfamily B

UniProt

P09341

Accession Number

P09341

Expression System

E.coli

Tag

N-Trx

Sequence

Ala35-Asn107

Field of Research

Immunology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.0 kDa

Observed Molecular Weight

25 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human