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Product details

SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified)

Catalog number SPD-C522w-1mg

Discontinued

Price for SPD-C522w-1mg

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Size / count 1 mg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike RBD, His Tag (BA.2.38/Omicron) (SPD-C522w) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417T, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.38).

Specifications

Catalog number
SPD-C522w-1mg
Size / count
1 mg
Dry ice
No
Hazardous
No
Discontinued
Yes
Background
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.7 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region
Arg 319 - Lys 537
Host
HEK 293
Target
Spike RBD
Conjugation
Unconjugated
Tag
C-10×His
Source
SARS-CoV-2
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD, His Tag (BA.2.38/Omicron) (Cat. No. SPD-C522w) with a linear range of 2-125 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
26.7 kDa
Shipping Conditions
Room Temperature
Storage Conditions
-20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified)?

The catalog number for SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified) is SPD-C522w-1mg.

Is SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified) in stock?

SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified)?

SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.38/Omicron) (MALS verified) used for?

SARS-CoV-2 Spike RBD, His Tag (BA.2.38/Omicron) (SPD-C522w) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417T, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)). The spike mutations are

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

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