QuicKey Pro Mouse IgG2b (Immunoglobulin G2b) ELISA Kit

Specifications

Catalog number

E-OSEL-M0015-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IgG2b

Cross Reactivity

This kit recognizes Mouse IgG2b in samples.No significant cross-reactivity or interference between mouse IgG2b and analogues was observed.

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2b. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG2b are added to the micro ELISA plate wells. Mouse IgG2b in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG2b.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgG2b. The concentration of Mouse IgG2b in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

11.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Ig2b concentrations in serum, plasma and other biological fluids.