Calsequestrin 2 Recombinant Rabbit Monoclonal Antibody [PSH03-16]

About this product

Calsequestrin is a calcium-binding protein that acts as a calcium buffer within the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. It also helps the sarcoplasmic reticulum store an extraordinarily high amount of calcium ions. Each molecule of calsequestrin can bind 18 to 50 Ca2+ ions. Sequence analysis has suggested that calcium is not bound in distinct pockets via EF-hand motifs, but rather via presentation of a charged protein surface. Two forms of calsequestrin have been identified. The cardiac form Calsequestrin-2 (CASQ2) is present in cardiac and slow skeletal muscle and the fast skeletal form Calsequestrin-1 (CASQ1) is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin is also secreted in the gut where it deprives bacteria of calcium ions.

Specifications

Catalog number

HA721944

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AA033488 antibody AW146219 antibody Calsequestrin 2 (cardiac muscle) antibody Calsequestrin 2 fast twitch cardiac muscle antibody Calsequestrin antibody Calsequestrin cardiac muscle isoform antibody Calsequestrin fast twitch cardiac muscle antibody Calsequestrin-2 antibody Calsequestrin2 antibody cardCSQ antibody Cardiac calsequestrin 2 antibody cardiac muscle isoform antibody CASQ 2 antibody CASQ2 antibody CASQ2_HUMAN antibody cCSQ antibody ESTM52 antibody FLJ26321 antibody FLJ93514 antibody PDIB2 antibody

Abbreviation

CASQ2

Swiss Prot

O14958 Human, O09161 Mouse, P51868 Rat

Cellular Locus

Sarcoplasmic reticulum lumen.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human Calsequestrin 2 aa 1-399 / 399.

Isotype

IgG

Clone

PSH03-16

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue

Positive Control

Mouse skeletal muscle tissue lysate, mouse heart tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000-1:10,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes