Phospho-VASP (S156) Recombinant Rabbit Monoclonal Antibody [JE45-91]

About this product

Vasodilator-stimulated phosphoprotein is a protein that in humans is encoded by the VASP gene. Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an N-terminal EVH1 domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions cell membranes. In the mid-region of the protein, family members have a proline-rich region that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Specifications

Catalog number

HA722145

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Vasodilator stimulated phosphoprotein antibody Vasodilator-stimulated phosphoprotein antibody VASP antibody VASP_HUMAN antibody

Abbreviation

VASP

Swiss Prot

P50552 Human, P70460 Mouse,

Cellular Locus

Cytoplasm, cytoskeleton, Cell junction, focal adhesion, tight junction, Cell projection, lamellipodium membrane, filopodium membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser156 of human VASP.

Isotype

IgG

Clone

JE45-91

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, A431 cells treated with 10μM forskolin for 1 hour, mouse spleen tissue, rat spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 40 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes