QuicKey Pro Rat VCAM-1/CD106 (Vascular Cell Adhesion Molecule 1) ELISA Kit

Specifications

Catalog number

E-OSEL-R0014-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

VCAM-1

UniProt

P29534

Cross Reactivity

This kit recognizes VCAM-1 in samples.No significant cross-reactivity or interference between VCAM-1 and analogues was observed.

Target

CD106; INCAM-100

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat VCAM-1/CD106 are added to the micro ELISA plate wells. Rat VCAM-1/CD106 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat VCAM-1/CD106.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VCAM-1/CD106. The concentration of Rat VCAM-1/CD106 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Detection Instrument

Microplate reader

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.