Human IL-10/Interleukin-10 High Sensitivity ELISA Kit PicoKine®

About this product

Human IL-10/Interleukin-10 High Sensitivity ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL10 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 0.5pg/ml.The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

HSEK0416

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

UNSPSC

41116127

HS Code

3822

Gene Name

IL10

Gene ID

3586

UniProt

P22301

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Capture Antibody

Monoclonal Antibody From Mouse

Detection Antibody

Monoclonal Antibody From Mouse

Applications

ELISA

Standard

10 ng/tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

0.78 pg/ml - 50 pg/ml

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.19 3.45 19.69 1.22 3.53 13.82 Standard deviation 0.082 0.094 1.365 0.036 0.132 0.843 CV (%) 6.9% 2.7% 6.9% 2.9% 3.7% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.19 3.45 19.69 1.22 3.53 13.82 Standard deviation 0.082 0.094 1.365 0.036 0.132 0.843 CV (%) 6.9% 2.7% 6.9% 2.9% 3.7% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1.19 3.45 19.69 1.22 3.53 13.82 Mean (pg/ml) 1.19 3.45 19.69 1.22 3.53 13.82 Standard deviation 0.082 0.094 1.365 0.036 0.132 0.843 Standard deviation 0.082 0.094 1.365 0.036 0.132 0.843 CV (%) 6.9% 2.7% 6.9% 2.9% 3.7% 6.1% CV (%) 6.9% 2.7% 6.9% 2.9% 3.7% 6.1%

Sensitivity

0.06pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human IL-10

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin 10

Protein Name

Interleukin-10

Sample Data

Concentration (pg/ml) 0 0.78 1.56 3.13 6.25 12.5 25 50 O.D. 0.029 0.12 0.21 0.383 0.725 1.235 2.044 3.25 Concentration (pg/ml) 0 0.78 1.56 3.13 6.25 12.5 25 50 O.D. 0.029 0.12 0.21 0.383 0.725 1.235 2.044 3.25 Concentration (pg/ml) 0 0.78 1.56 3.13 6.25 12.5 25 50 Concentration (pg/ml) 0 0.78 1.56 3.13 6.25 12.5 25 50 O.D. 0.029 0.12 0.21 0.383 0.725 1.235 2.044 3.25 O.D. 0.029 0.12 0.21 0.383 0.725 1.235 2.044 3.25