Mouse IL-28A/B ELISA Kit PicoKine®

About this product

Mouse IL-28A/B ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse IL-28A/B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2386

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-28 (IL-28) is a cytokine that comes in two isoforms, IL-28A and IL-28B, and plays a role in immune defense against viruses, including the induction of an "antiviral state" by turning on Mx proteins, 2',5'-oligoadenylate synthetase as well as ISGF3G (Interferon Stimulated Gene Factor 3) .

Synonyms

IL28A, IFNL2a, IFNL3a, IL-28A; IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4

UNSPSC

41116127

HS Code

3822

Gene Name

Ifnl2/Ifnl3

Gene ID

282616/282617

UniProt

Q4VK74/Q8CGK6

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard:E.coli, Immunogen sequence: Asp20-Val193

Capture Antibody

Polyclonal Antibody From Goat

Detection Antibody

Polyclonal Antibody From Goat

Applications

ELISA

Standard

10 ng/tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/ml - 1,000 pg/ml

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 86 267 25 99 256 Standard deviation 1.54 4.39 14.10 1.08 7.62 19.87 CV (%) 5.3% 5.1% 5.3% 4.3% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 86 267 25 99 256 Standard deviation 1.54 4.39 14.10 1.08 7.62 19.87 CV (%) 5.3% 5.1% 5.3% 4.3% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 86 267 25 99 256 Mean (pg/ml) 29 86 267 25 99 256 Standard deviation 1.54 4.39 14.10 1.08 7.62 19.87 Standard deviation 1.54 4.39 14.10 1.08 7.62 19.87 CV (%) 5.3% 5.1% 5.3% 4.3% 7.7% 7.8% CV (%) 5.3% 5.1% 5.3% 4.3% 7.7% 7.8%

Sensitivity

<4 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse IL-28A/B

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interferon lambda 2/interferon lambda 3

Protein Name

Interferon lambda-2; Interferon lambda-3

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.052 0.108 0.202 0.398 0.772 1.346 2.397 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.052 0.108 0.202 0.398 0.772 1.346 2.397 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.052 0.108 0.202 0.398 0.772 1.346 2.397 O.D. 0.003 0.052 0.108 0.202 0.398 0.772 1.346 2.397

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.