Sphingomyelin (SM) Fluorometric Assay Kit

Specifications

Catalog number

E-BC-F079

Size / count

96 T (40 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Fluorescence method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Sphingomyelin (SM) is widely present in cell membranes and plasma lipoproteins. Metabolism of SM is regulated by sphingomyelin synthase and sphingomyelase, whose metabolites include ceramide, sphingosinol and choline phosphate, which are important second messengers. Studies have shown that SM and its metabolites play an important role in liver, lung, and cardiovascular diseases. The principle of this kit is that SM is catalyzed by sphingomyelin enzyme, and its products generate fluorescent substances with the probe under the action of other enzymes. By substituting the fluorescence value into the standard curve, SM content in the sample can be calculated.

Assay Performance Time

1 h 30 min

Sample Type

Serum; Plasma; Animal tissue; Cell

Detection Range

0.35-40 μmol/L

Sensitivity

0.35 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

6.0-10.0%

Intra CV

4.9-5.1%

Detection Instrument

Fluorescence microplate reader