Mouse PAI-1 ELISA Kit PicoKine®

About this product

Mouse PAI-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse PAI-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 0.3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2393

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots) . It is a serine protease inhibitor (serpin) protein (SERPINE1) . PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22) .1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage) . PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

Synonyms

PAI; PAI 1; PAI1; PAI-1; PLANH1; Serpin E1; SERPINE1; PAI-1

UNSPSC

41116127

HS Code

3822

Gene Name

PAI-1

Gene ID

18787

UniProt

P22777

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NSO; Immunogen sequence: T23-P402

Capture Antibody

Polyclonal Antibody From Goat

Detection Antibody

Polyclonal Antibody From Goat

Applications

ELISA

Standard

1 ng/tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

7.8 pg/ml - 500 pg/ml

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 33 248 12 39 197 Standard deviation 0.40 1.53 15.38 0.71 1.44 14.01 CV (%) 2.7% 4.7% 6.2% 5.9% 3.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 33 248 12 39 197 Standard deviation 0.40 1.53 15.38 0.71 1.44 14.01 CV (%) 2.7% 4.7% 6.2% 5.9% 3.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 33 248 12 39 197 Mean (pg/ml) 15 33 248 12 39 197 Standard deviation 0.40 1.53 15.38 0.71 1.44 14.01 Standard deviation 0.40 1.53 15.38 0.71 1.44 14.01 CV (%) 2.7% 4.7% 6.2% 5.9% 3.7% 7.1% CV (%) 2.7% 4.7% 6.2% 5.9% 3.7% 7.1%

Sensitivity

<0.3pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse PAI-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Serpin family E member 1

Protein Name

Plasminogen activator inhibitor 1

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.008 0.087 0.155 0.266 0.528 0.905 1.562 2.092 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.008 0.087 0.155 0.266 0.528 0.905 1.562 2.092 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.008 0.087 0.155 0.266 0.528 0.905 1.562 2.092 O.D. 0.008 0.087 0.155 0.266 0.528 0.905 1.562 2.092

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.