Human Irisin/FNDC5 ELISA Kit PicoKine®

About this product

Human Irisin/FNDC5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human Irisin/FNDC5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2388

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms.

Synonyms

CNC, NCX1

UNSPSC

41116127

HS Code

3822

Gene Name

FNDC5

Gene ID

252995

UniProt

Q8NAU1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO, Immunogen sequence: Asp32-Glu143

Capture Antibody

Polyclonal Antibody From Goat

Detection Antibody

Polyclonal Antibody From Goat

Applications

ELISA

Standard

10 ng/tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

156 pg/ml - 10,000 pg/ml

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 893 3980 278 1073 3141 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 893 3980 278 1073 3141 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 893 3980 278 1073 3141 Mean (pg/ml) 162 893 3980 278 1073 3141 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7% CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7%

Sensitivity

<20 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Irisin

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Fibronectin type III domain containing 5

Protein Name

Fibronectin type III domain-containing protein 5

Sample Data

Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969 Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969 Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.