LAG-3 Recombinant Rabbit Monoclonal Antibody [PSH0-44]

About this product

Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene. LAG3, which was discovered in 1990 and was designated CD223 (cluster of differentiation 223) after the Seventh Human Leucocyte Differentiation Antigen Workshop in 2000, is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders. In soluble form it is also being developed as a cancer drug in its own right. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function. Fibrinogen-like protein1 FGL1, a liver-secreted protein, is another (major) LAG3 functional ligand independent of MHC-II. LAG3 also helps maintain CD8+ T cells in a tolerogenic state and, working with PD-1, helps maintain CD8 exhaustion during chronic viral infection. LAG3 is known to be involved in the maturation and activation of dendritic cells.

Specifications

Catalog number

HA721346

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CD223 antibody CD223 antigen antibody FDC protein antibody LAG-3 antibody Lag3 antibody LAG3_HUMAN antibody Lymphocyte activating 3 antibody Lymphocyte activation gene 3 antibody Lymphocyte activation gene 3 protein antibody Protein FDC antibody

Abbreviation

LAG3, FDC

Swiss Prot

P18627 Human, Q61790 Mouse

Cellular Locus

Cell membrane; Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human LAG-3 aa 1-500 / 525.

Isotype

IgG

Clone

PSH0-44

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

NIH/3T3 transfected with LAG3 cell lysate, human tonsil tissue, NIH/3T3 transfected with LAG3.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:200 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 57 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes