Bovine IL-2 ELISA Kit PicoKine®

About this product

Bovine IL-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Bovine IL-2 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA or citrate) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2389

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Synonyms

T-cell growth factor (TCGF)

UNSPSC

41116127

HS Code

3822

Gene Name

IL2

Gene ID

280822

UniProt

P05016

Reactivity

Bovine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-T55

Capture Antibody

Polyclonal Antibody From Goat

Detection Antibody

Polyclonal Antibody From Goat

Applications

ELISA

Standard

10 ng/tube

Sample Type

Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA or citrate) .

Detection Range

15.6 pg/ml - 1,000 pg/ml

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 77 315 16 107 330 Standard deviation 0.66 2.92 18.49 1.21 4.80 18.12 CV (%) 3.0% 3.8% 5.9% 7.5% 4.5% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 77 315 16 107 330 Standard deviation 0.66 2.92 18.49 1.21 4.80 18.12 CV (%) 3.0% 3.8% 5.9% 7.5% 4.5% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 22 77 315 16 107 330 Mean (pg/ml) 22 77 315 16 107 330 Standard deviation 0.66 2.92 18.49 1.21 4.80 18.12 Standard deviation 0.66 2.92 18.49 1.21 4.80 18.12 CV (%) 3.0% 3.8% 5.9% 7.5% 4.5% 5.5% CV (%) 3.0% 3.8% 5.9% 7.5% 4.5% 5.5%

Sensitivity

<4 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant bovine IL-2

Gene Name Synonym

Interleukin 2

Protein Name

Interleukin-2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.091 0.137 0.237 0.435 0.76 1.399 2.104 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.091 0.137 0.237 0.435 0.76 1.399 2.104 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.091 0.137 0.237 0.435 0.76 1.399 2.104 O.D. 0.011 0.091 0.137 0.237 0.435 0.76 1.399 2.104

Sample Activation Procedure

Heparin, EDTA or citrate

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.