Bovine IFN-gamma ELISA Kit PicoKine®

About this product

Bovine IFN-gamma ELISA Kit PicoKine® (96 Tests) . Quantitate Bovine IFN-gamma in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA or citrate) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2390

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Synonyms

Interferon gamma; BoIFNG; IFN-gamma

UNSPSC

41116127

HS Code

3822

Gene Name

IFNG

Gene ID

281237

UniProt

P07353

Reactivity

Bovine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G24-M166

Capture Antibody

Polyclonal Antibody From Goat

Detection Antibody

Polyclonal Antibody From Goat

Applications

ELISA

Standard

10 ng/tube

Sample Type

Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA or citrate) .

Detection Range

15.6 pg/ml - 1,000 pg/ml

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 80 475 17 80 250 Standard deviation 1.31 5.81 15.53 0.60 4.87 19.90 CV (%) 6.2% 7.3% 3.3% 3.6% 6.1% 8.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 80 475 17 80 250 Standard deviation 1.31 5.81 15.53 0.60 4.87 19.90 CV (%) 6.2% 7.3% 3.3% 3.6% 6.1% 8.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 21 80 475 17 80 250 Mean (pg/ml) 21 80 475 17 80 250 Standard deviation 1.31 5.81 15.53 0.60 4.87 19.90 Standard deviation 1.31 5.81 15.53 0.60 4.87 19.90 CV (%) 6.2% 7.3% 3.3% 3.6% 6.1% 8.0% CV (%) 6.2% 7.3% 3.3% 3.6% 6.1% 8.0%

Sensitivity

<4 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant bovine IFN-gamma

Gene Name Synonym

Interferon gamma

Protein Name

Interferon gamma

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.059 0.102 0.166 0.28 0.524 1.002 1.904 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.059 0.102 0.166 0.28 0.524 1.002 1.904 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.059 0.102 0.166 0.28 0.524 1.002 1.904 O.D. 0.018 0.059 0.102 0.166 0.28 0.524 1.002 1.904

Sample Activation Procedure

Heparin, EDTA or citrate

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.