Human IFN-γ (Interferon Gamma) ELISPOT Kit

Specifications

Catalog number

ESP-H0002-01

Size / count

96 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IFN-γ

UniProt

P01579

Cross Reactivity

This kit recognizes Human IFN-γ in samples.No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.

Target

IFNG; IFG; IFI; Type II Interferon

Applications

ELISPOT

Field of Research

Cell Biology; Immunology; Neuroscience

Assay Principle

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IFN-γ antibodies. During the experiment, cell suspension and stimulants are added first, and IFN-γ secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IFN-γ antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IFN-γ antibodies will bind specifically to the IFN-γ which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IFN-γ secreting cells.

Assay Performance Time

3 h 30 min

Sample Type

Suspension cell

Precision

Both intra-CV and inter-CV are < 10%.

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Species

Human

Recommended Usage

This ELISPOT kit is designed for the detection of the frequency of human IFN-γ secreting cells.