Human TNF-α (Tumor Necrosis Factor Alpha) ELISPOT Kit

Specifications

Catalog number

ESP-H0010-01

Size / count

96 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TNF-α

UniProt

P01375

Cross Reactivity

This kit recognizes Human TNF-α in samples.No significant cross-reactivity or interference between Human TNF-α and analogues was observed.

Target

DIF; TNF-alpha; TNFA; TNFSF2

Applications

ELISPOT

Field of Research

Cell Biology; Immunology

Assay Principle

This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human TNF-α antibodies. During the experiment, cell suspension and stimulants are added first, and TNF-α secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human TNF-α antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human TNF-α antibodies will bind specifically to the TNF-α which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human TNF-α secreting cells.

Assay Performance Time

3 h 30 min

Sample Type

Suspension cell

Precision

Both intra-CV and inter-CV are < 10%.

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Species

Human

Recommended Usage

This ELISPOT kit is designed for the detection of the frequency of human TNF-α secreting cells.