Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit

Q: What is the catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

The catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is ESP-H0010S.

Q: What is the estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

Estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is approximately 15 business day(s), depending on your location.

Q: Where can I find the safety data sheet (SDS/MSDS) for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

The safety data sheet for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is available here: https://789.bio/eb/ZfVN0z03.

Q: What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

Q: How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

Q: What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

Catalog number ESP-H0010S-01

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Price for ESP-H0010S-01

Size / count 96 Tests

Specifications

Catalog number: ESP-H0010S-01
Size / count: 96 Tests
Dry ice: No
Hazardous: No
Discontinued: No
Abbreviation: TNF-α
UniProt: P01375
Cross Reactivity: This kit recognizes Human TNF-α in samples.No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
Target: DIF, TNF-alpha, TNFA, TNFSF2
Applications: ELISPOT
Field of Research: Cell Biology; Immunology
Assay Principle: This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human TNF-α antibodies. During the experiment, cell suspension and stimulants are added first, and TNF-α secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human TNF-α antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human TNF-α antibodies will bind specifically to the TNF-α which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human TNF-α secreting cells.
Assay Performance Time: 3 h 30 min
Sample Type: Suspension cell
Precision: Both intra-CV and inter-CV are < 10%.
Shipping Conditions: Ice bag
Storage Conditions: 2-8°C,12 months
Species: Human
Recommended Usage: This ELISPOT kit is designed for the detection of the frequency of human TNF-α secreting cells.

Frequently Asked Questions

What is the catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

The catalog number for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is ESP-H0010S.

What sizes are available for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is available in the following size(s): 96 Tests, 5x 96 Tests.

How many pack sizes does Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit come in?

Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is available in 2 different pack sizes.

Is Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit in stock?

Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is manufactured by Elabscience.

What is the estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

Estimated delivery time for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit?

The safety data sheet for Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit is available here: https://789.bio/eb/ZfVN0z03.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.