eIF3A Recombinant Rabbit Monoclonal Antibody [JE58-60]

About this product

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

Specifications

Catalog number

HA721375

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

centrosomin homolog antibody cytoplasmic protein p167 antibody eIF 3 theta antibody eIF-3-theta antibody EIF3 antibody eIF3 p167 antibody eIF3 p170 antibody eIF3 p180 antibody EIF3 p180 subunit antibody eIF3 p185 antibody eIF3 theta antibody eIF3a antibody EIF3A_HUMAN antibody EIF3S10 antibody eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) antibody eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) antibody eukaryotic translation initiation factor 3 subunit 10 170kDa antibody Eukaryotic translation initiation factor 3 subunit 10 antibody eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa antibody Eukaryotic translation initiation factor 3 subunit A antibody OTTHUMP00000020589 antibody P167 antibody p180 antibody p185 antibody TIF32 antibody

Abbreviation

EIF3A

Swiss Prot

Q14152 Human, P23116 Mouse, Q1JU68 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human eIF3A aa 275-324 / 1,382.

Isotype

IgG

Clone

JE58-60

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

293T cell lysate, A431 cell lysate, Jurkat cell lysate, Raji cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, L6 cell lysate, PC-12 cell lysate, mouse brain tissue, rat brain tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 167 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes