QuicKey Pro Human PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit

Specifications

Catalog number

E-OSEL-H0033-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

PIIINP

UniProt

P02461

Cross Reactivity

This kit recognizes PIIINP in samples.No significant cross-reactivity or interference between PIIINP and analogues was observed.

Target

P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIIINP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PIIINP are added to the micro ELISA plate wells. Human PIIINP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PIIINP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIIINP. The concentration of Human PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.09 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human PIIINP concentrations in serum, plasma and other biological fluids.