CD32B Mouse Monoclonal Antibody [A9C11]

About this product

Fc fragment of IgG receptor IIb (coded by FCGR2B gene) is a low affinity inhibitory receptor for the Fc region of immunoglobulin gamma (IgG). FCGR2B participates in the phagocytosis of immune complexes and in the regulation of antibody production by B lymphocytes. The receptor inhibits the functions of activating FcγRs, such as phagocytosis and pro-inflammatory cytokine release, mainly by clustering of FCGR2B with different activating FCGR receptors or with the BCR by immune complexes. The phosphorylated ITIM of FcγRIIB recruits the inositol phosphatases SHIP1 and SHIP2, which inhibit the Ras activation, downregulate MAPK activity and reduce PLCγ function and lead to decreased activation of PKC. Inhibition of the MAP kinase pathway, together with the anti-apoptotic kinase Akt can negatively affect proliferation and survival of the cells. FCGR2B regulates B cell activation by increasing the BCR activation threshold and suppressing B cell mediated antigen presentation to T cells through the ITIM-dependent inhibitory mechanism. Ligation of FCGR2B on B cells downregulates antibody production, prevents the membrane organization of BCR and CD19 and promotes apoptosis. Co-ligation of FCGR2B on dendritic cells inhibits maturation and blocks cell activation. .The negative regulatory role of the FCGRIIB molecule is not limited to BCR-induced B-cell activation, but is also functional on other B-cell activation pathways mediated by CD40 and IL-4. FCGR2B expression on follicular dendritic cells (FDCs) is important for capturing the antigen-containing immune complexes which are essential for the germinal centre response. FCGR2B is present on non-leukocyte cells including airway smooth muscle and liver sinusoidal endothelial cells, where small immune complexes are internalized inhibiting the pro-inflammatory signalling.

Specifications

Catalog number

HA601121

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CD32 antibody CD32 antigen antibody CD32B antibody CDw32 antibody Fc fragment of IgG, low affinity II, receptor for (CD32) antibody Fc fragment of IgG, low affinity IIb, receptor antibody Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody Fc fragment of IgG, low affinity IIb, receptor for antibody Fc gamma receptor IIB antibody Fc gamma RII antibody Fc gamma RIIB antibody Fc-gamma RII antibody Fc-gamma RII-b antibody Fc-gamma-RIIb antibody FCG2 antibody FCG2B_HUMAN antibody Fcgr2 antibody FCGR2B antibody FCRII antibody FcRII-b antibody IGFR2 antibody IgG Fc receptor II beta antibody IgG Fc receptor II-b antibody Low affinity immunoglobulin gamma Fc region receptor II antibody Low affinity immunoglobulin gamma Fc region receptor II-b antibody Ly-17 antibody Lym 1 antibody Lymphocyte antigen 17 antibody

Abbreviation

FCGR2B, CD32, FCG2, IGFR2

Swiss Prot

P31994 Human

Cellular Locus

Cell membrane.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within human aa 1-250 / 310.

Isotype

IgG1

Clone

A9C11

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Daudi cell lysate, Raji cell lysate, HL-60 cell lysate, human placenta tissue lysate, human tonsil tissue, human liver tissue, human colon carcinoma tissue, human placenta tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 34 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No