GABA Transporter 1 / GAT 1 Recombinant Rabbit Monoclonal Antibody [JE34-61]

About this product

GABA transporter 1 (GAT1) also known as sodium- and chloride-dependent GABA transporter 1 is a protein that in humans is encoded by the SLC6A1 gene and belongs to the solute carrier 6 (SLC6) family of transporters. It mediates gamma-aminobutyric acid's translocation from the extracellular to intracellular spaces within brain tissue and the central nervous system as a whole. GAT1 is a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft by shuttling it to presynaptic neurons (where GABA can be recycled) and astrocytes (where GABA can be broken down). GABA Transporter 1 uses energy from the dissipation of a Na+ gradient, aided by the presence of a Cl− gradient, to translocate GABA across CNS neuronal membranes. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl−: 1 GABA. The presence of a Cl−/Cl− exchange is also proposed because the Cl− transported across the membrane does not affect the net charge. GABA is also the primary inhibitory neurotransmitter in the cerebral cortex and has the highest level of expression within it. The GABA affinity (Km) of the mouse isoform of GAT1 is 8 μM. In the brain of a mature mammal, glutamate is converted to GABA by the enzyme glutamate decarboxylase (GAD) along with the addition of vitamin B6. GABA is then packed and released into the post-synaptic terminals of neurons after synthesis. GABA can also be used to form succinate, which is involved in the citric acid cycle. Vesicle uptake has been shown to prioritize newly synthesized GABA over preformed GABA, though the reasoning behind this mechanism is currently not completely understood. The regulation of the modular functioning of GATs is highly dependent on a multitude of second messengers and synaptic proteins.

Specifications

Catalog number

HA721496

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

GABATHG antibody GABATR antibody GABT 1 antibody GABT1 antibody GAT-1 antibody GAT1 antibody SC6A1_HUMAN antibody Slc6a1 antibody Sodium and chloride dependent GABA transporter 1 antibody Sodium- and chloride-dependent GABA transporter 1 antibody Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody Solute carrier family 6 member 1 antibody

Abbreviation

SLC6A1, GABATR, GABT1, GAT1

Swiss Prot

P30531 Human, P31648 Mouse, P23978 Rat

Cellular Locus

Cell membrane, Presynapse.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-52.

Isotype

IgG

Clone

JE34-61

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Positive Control

Mouse cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, human liver tissue, mouse cerebellum tissue, mouse retina tissue, rat brain tissue, rat retina tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:500-1:2,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 67 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes