Recombinant Phospho-AKT (Ser473) Monoclonal Antibody — image 1 of 5

Product details

Recombinant Phospho-AKT (Ser473) Monoclonal Antibody

Catalog number AN300086L-01

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Price for AN300086L-01

Size / count 20 µL

Specifications

Catalog number: AN300086L-01
Size / count: 20 µL
Dry ice: No
Hazardous: No
Discontinued: No
Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Abbreviation: AKT1
UniProt: P31749
Host: Rabbit
Reactivity: Human
Immunogen: A synthetic phosphopeptide corresponding to residues around Ser473 of human AKT.
Target: CWS; MGC; AKT serine/threonine kinase; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1; AKT 1; AKT serine/threonine kinase 1; AKT1_HUMAN; MGC99656; Protein kinase B; Protein Kinase B Alpha; Proto-oncogene c-Akt; RAC Alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; AKT; AKT serine/threonine kinase 1; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Clonality: Monoclonal
Clone: 11A2
Conjugation: Unconjugated
Applications: WB; IHC-P
Field of Research: Signal Transduction; Epigenetics and Nuclear Signaling; Cancer; Metabolism; Neuroscience
Purification: Protein A
Concentration: 1 mg/mL
Dilution: WB 1:1000-1:200000; IHC-P 1:100-1:400
Buffer: 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5
Shipping Conditions: Ice bag
Storage Conditions: This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight: 55 kDa
Observed Molecular Weight: 55 kDa
Isotype: IgG

Frequently Asked Questions

What is the catalog number for Recombinant Phospho-AKT (Ser473) Monoclonal Antibody?

The catalog number for Recombinant Phospho-AKT (Ser473) Monoclonal Antibody is AN300086L.

What sizes are available for Recombinant Phospho-AKT (Ser473) Monoclonal Antibody?

Recombinant Phospho-AKT (Ser473) Monoclonal Antibody is available in the following size(s): 20 µL, 100 µL.

How many pack sizes does Recombinant Phospho-AKT (Ser473) Monoclonal Antibody come in?

Recombinant Phospho-AKT (Ser473) Monoclonal Antibody is available in 2 different pack sizes.

Is Recombinant Phospho-AKT (Ser473) Monoclonal Antibody in stock?

Recombinant Phospho-AKT (Ser473) Monoclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Recombinant Phospho-AKT (Ser473) Monoclonal Antibody?

Recombinant Phospho-AKT (Ser473) Monoclonal Antibody is manufactured by Elabscience.

What is the estimated delivery time for Recombinant Phospho-AKT (Ser473) Monoclonal Antibody?

Estimated delivery time for Recombinant Phospho-AKT (Ser473) Monoclonal Antibody is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Recombinant Phospho-AKT (Ser473) Monoclonal Antibody?

The safety data sheet for Recombinant Phospho-AKT (Ser473) Monoclonal Antibody is available here: https://789.bio/eb/lSJt0vKP.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.