Recombinant Phospho-Stat5 (Tyr694) Monoclonal Antibody

Specifications

Catalog number

AN300135L-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L2/BCL-X (L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.

Abbreviation

STAT5a

UniProt

P42229

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues around Tyr694 of human Phospho-Stat5.

Target

MGF; STAT5; STAT5A; STA5A; Mammary gland factor; Signal transducer and activator of transcription 5A; STAT 5; STAT 5A

Clonality

Monoclonal

Clone

3G5

Conjugation

Unconjugated

Applications

WB; IHC-P

Field of Research

Signal Transduction; Epigenetics and Nuclear Signaling; Cancer; Metabolism

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:1000-1:10000; IHC-P 1:50-1:200

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

90 kDa

Observed Molecular Weight

100 kDa

Isotype

IgG