Recombinant Phospho-SMAD2 (Ser465, 467) Monoclonal Antibody

Specifications

Catalog number

AN300146L-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 2 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Abbreviation

Smad2

UniProt

Q15796

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to the residues around (Ser465, 467) of Human Phospho-SMAD2

Target

MADH; MADR; hMAD; SMAD; hSMAD; JV18; JV18-1; MADH2; MADR2; hMAD-2; hSMAD2; Smad2; Drosophila; homolog of; hSMAD family member 2; JV181; MAD; MAD homolog 2; MAD Related Protein 2; Mad-related protein 2; MGC22139; MGC34440; Mother against DPP homolog 2; Mothers against decapentaplegic; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; OTTHUMP00000163489; Sma and Mad related protein 2; Sma- and Mad-related protein 2 MAD; SMAD 2; SMAD family member 2

Clonality

Monoclonal

Clone

4B12

Conjugation

Unconjugated

Applications

WB

Field of Research

Signal Transduction; Epigenetics and Nuclear Signaling; Stem Cells; Cancer; Kits; Lysates; Other; Metabolism

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52 kDa

Observed Molecular Weight

60 kDa

Isotype

IgG