Recombinant Phospho-Chk2 (Thr68) Monoclonal Antibody

Specifications

Catalog number

AN300151L-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Abbreviation

CHEK2

UniProt

O96017

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues around Thr68 of the Human Chk2

Target

RAD; LFS; CHK; CDS; hCds; CHEK; HuCds; CDS1; CHK2; HuCds1; LFS2; PP1425; RAD53; hCds1; CHEK2; CDS 1; Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; CHEK 2; Chk 2; CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe) ; HuCds 1; LFS 2; RAD 53; Rad53 homolog; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2

Clonality

Monoclonal

Clone

5B2

Conjugation

Unconjugated

Applications

WB

Field of Research

Epigenetics and Nuclear Signaling; Cancer

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:1000-1:10000

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

61 kDa

Observed Molecular Weight

61 kDa

Isotype

IgG