Recombinant AGO2/Argonaute 2/EIF2C2 Monoclonal Antibody

Specifications

Catalog number

AN300165P-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA) -directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

Abbreviation

AGO2

UniProt

Q8CJG0

Host

Rabbit

Reactivity

Mouse

Immunogen

Recombinant Mouse AGO2 protein

Target

AGO; Kiaa; Gerp; Eif2c; mKIAA; ENSMUSG;1110029L17Rik;2310051F07Rik; AI225898; AL022874; AW546247; ENSMUSG00000072493; Gerp95; Gm10365; mKIAA4215; Eif2c2; Kiaa4215; Ago2

Clonality

Monoclonal

Clone

5H4

Conjugation

Unconjugated

Applications

IHC-P; IP

Purification

Protein A

Concentration

1 mg/mL

Dilution

IHC-P 1:100-1:500; IP 1-4 μL/mg of lysate

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Isotype

IgG