Recombinant SOD2 Monoclonal Antibody

Specifications

Catalog number

AN300260P

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Superoxide Dismutases (SODs), originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 2 (SOD2), also known as Manganese (Mn) SOD, mitochondrial SOD, and IPO-B, is an intramitochondrial 22 kDa homotetramer. Each SOD2 monomer binds one Mn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD2 shares only 23% and 17% sequence identity with SOD1 and SOD3, respectively. SOD2 is, however, well conserved from species to species and shares 90% and 87% homology with mouse and rat SOD2, respectively. Oxidative stress has been implicated in many diseases and the chief source of reactive oxygen species within the cell is the mitochondrion. SOD2 is a free radical scavenging enzyme that protects against damage from superoxide produced as a byproduct of oxidative phosphorylation. SOD2 is required for normal biologic function of tissues by maintaining the integrity of mitochondrial enzymes susceptible to inactivation by superoxide. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.

Abbreviation

SOD2

UniProt

P04179

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human SOD2 protein

Target

SOD; MVCD; superoxide dismutase; IPO-B; IPOB; MNSOD; MVCD6; Mn-SOD; SOD2; Superoxide Dismutase [Mn] Mitochondrial; superoxide dismutase 2; IPOB; IPO-B; MNSOD; Mn-SOD; MVCD6; superoxide dismutase 2; EC:1.15.1.1; mitochondrial; Superoxide dismutase [Mn]

Clonality

Monoclonal

Clone

11A12

Conjugation

Unconjugated

Applications

WB; IP

Field of Research

Cell Biology; Signal Transduction; Neuroscience; Cardiovascular; Cancer; Metabolism

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:2000-1:10000; IP 1-4 μL/mg of lysate

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25 kDa

Observed Molecular Weight

25 kDa

Isotype

IgG