Recombinant SARS-CoV-2 NSP9 protein

Specifications

Catalog number

PKSV030326-01

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

During infection of human cells, SARS-CoV Non-structural protein 9 (Nsp9SARS) was found to be essential for replication. Homologs of the Nsp9 protein have been identified in numerous coronaviruses, including SARS-Cov-2 (Nsp9COV19), human coronavirus 229E (Nsp9HcoV), avian infectious bronchitis virus (Nsp9IBV), porcine epidemic diarrhea virus (Nsp9PEDV), and porcine delta virus (Nsp9PDCoV) . Nsp9SARS has been shown to have modest affinity for long oligonucleotides with binding thought to be dependent on oligomerization state. Nsp9SARS dimerizes in solution via a conserved α-helical “GxxxG” motif. Disruption of key residues within this motif reduces both RNA binding and SARS-CoV viral replication. The mechanism of RNA binding within the Nsp9 protein family is not understood as these proteins have an unusual structural fold not previously seen in RNA-binding proteins. The fold's Greek-key motif exhibits topological similarities with Oligonucleotide/oligosaccharide binding proteins (OB-fold), but such vestiges have proven insufficient to provide clear insight into Nsp9 function. As a consequence of the weak affinity of Nsp9SARS for long oligonucleotide stretches it was suggested that the natural RNA substrate may instead be conserved features at the 3′ end of the viral-genome (the stem-loop II RNA-motif) . Furthermore, potential direct interactions with the co-factors of the RNA polymerase have been reported. However, it remains to be determined how the oligonucleotide-binding activity of Nsp9 proteins promotes viral replication during infection.

Synonyms

SARS-CoV 2 nsp9; Single-stranded RNA-binding protein

UniProt

P0DTC2

Accession Number

QHD43415.1

Expression System

E. coli

Tag

N-His

Sequence

Asn4141-Gln4253

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.7 kDa

Observed Molecular Weight

13 - 14 kDa

Formulation

Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.

Species

SARS-CoV-2