Recombinant Human ISG15 Protein (Sumo Tag)

Specifications

Catalog number

PDEH100478-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ISG15 (interferon-induced 15 kDa protein, also known as IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type-I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2-78 and 79-157) and a propeptide (aa 158-165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature Human ISG15 is 66% aa identical to Mouse ISG15.

Abbreviation

ISG15

Synonyms

G1P2; hUCRP; IFI 15; IFI15; Interferon alpha inducible protein; Interferon induced protein 15; Interferon induced protein IFI-15K; Interferon stimulated protein 15 kDa; Interferon; alpha-inducible protein (clone IFI-15K) ; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; Interferon-induced 17-kDa/15-kDa protein; IP17; ISG 15; ISG15; ISG15 ubiquitin like modifier; ISG15; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15; UCRP

UniProt

P05161

Accession Number

P05161

Expression System

E.coli

Tag

N-Sumo

Sequence

Met1-Gly157

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.2 kDa

Observed Molecular Weight

33 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human