Recombinant Human STAR Protein (Sumo Tag)

Specifications

Catalog number

PDEH100498-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Abbreviation

STAR

Synonyms

Cholesterol trafficker; Luteinizing hormone induced protein; mitochondrial; Mitochondrial steroid acute regulatory protein; StAR; StAR related lipid transfer (START) domain containing 1; STAR; StARD1; START domain containing 1; START domain containing protein 1; START domain-containing protein 1; Steroid acute regulatory protein; Steroidogenic acute regulator; Steroidogenic acute regulatory protein; Steroidogenic acute regulatory protein mitochondrial

UniProt

P49675

Accession Number

P49675

Expression System

E.coli

Tag

N-Sumo

Sequence

Met1-Cys285

Field of Research

Signal Transduction; Cancer; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44.2 kDa

Observed Molecular Weight

45-50 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human