Recombinant Human BMP-2 Protein (Gst Tag)

Specifications

Catalog number

PDEH100524-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients.

Abbreviation

BMP-2

Synonyms

BDA2; BMP-2; BMP-2A; Bmp2; BMP2; BMP2A; Bone morphogenetic protein 2; Bone morphogenetic protein 2A

UniProt

P12643

Accession Number

P12643

Expression System

E.coli

Tag

N-Gst

Sequence

Gln283-Arg396

Field of Research

Cancer; Cardiovascular; Signal Transduction; Stem Cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

38 kDa

Observed Molecular Weight

42 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human