Recombinant Human AREG Protein (Trx Tag)

Specifications

Catalog number

PDEH100533-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) . The protein interacts with an the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with an a psoriasis-like skin phenotype, and is also associated with an other pathological disorders, including various types of cancers and inflammatory conditions.

Abbreviation

AREG

Synonyms

A REG; Amphiregulin; Amphiregulin B; AR; AREG; AREG; AREGB; Colorectum cell derived growth factor; Colorectum cell-derived growth factor; CRDGF; MGC13647; Schwannoma derived growth factor; SDGF

UniProt

P15514

Accession Number

P15514

Expression System

E.coli

Tag

N-Trx

Sequence

Ser101-Lys184

Field of Research

Cancer; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

29 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human