Recombinant Human S100A1 Protein (Trx Tag)

Specifications

Catalog number

PDEH100557-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600) . In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes (PubMed:23351007) . These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290) . Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600) . Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole.

Abbreviation

S100A1

Synonyms

Bpb; NEF; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 alpha; S100 beta; S100 calcium binding protein A1; S100 calcium binding protein B; S100 calcium-binding protein A1; S100 protein alpha polypeptide; S100A; s100a1; S100B; S100beta; S10A1

UniProt

P23297

Accession Number

P23297

Expression System

E.coli

Tag

N-Trx

Sequence

Met1-Ser94

Field of Research

Cancer; Neuroscience; Signal Transduction; Tags and Cell Markers

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.3 kDa

Observed Molecular Weight

30 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human