Recombinant Human CXCL7/NAP-2 Protein (Sumo Tag)

Specifications

Catalog number

PDEH100572-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pro-platelet basic protein (PPBP) is also known as Chemokine (C-X-C motif) ligand 7 (CXCL7) and nucleosome assembly protein (Nap-2) . Nap-2 / PPBP / CXCL7 is released in large amounts from platelets following their activation and is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. Nap-2 / PPBP / CXCL7 has been shown to stimulate various cellular processes including DNA synthesis , mitosis , glycolysis , intracellular cAMP accumulation , prostaglandin E2 secretion , and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Nap-2 is a ligand for CXCR1 and CXCR2 , and Nap-2 , Nap-2 (73), Nap-2 (74), Nap-2 (1-66), and most potent Nap-2 (1-63) are chemoattractants and activators for neutrophils.

Abbreviation

CXCL7/NAP-2

Synonyms

B-TG; CTAP; CXCL; SCYB; C-X-C Motif Chemokine; MDGFSmall-Inducible Cytokine B; LA-PF; NAP; PPBP; B-TG1; Beta-TG; CTAP-III; CTAP3; CTAPIII; CXCL7; LA-PF4; LDGF; MDGF; NAP-2; PBP; SCYB7; TC1; TC2; TGB; TGB1; THBGB; THBGB1; C-X-C Motif Chemokine 7; Leukocyte-Derived Growth Factor; Macrophage-Derived Growth Factor; MDGFSmall-Inducible Cytokine B7; NAP2; Platelet Basic Protein; βTG; B TG1; Beta TG; Beta thromboglobulin; beta-1; Chemokine (C X C motif) ligand 7; Connective tissue activating peptide III; CTAP 3; CTAP III; CTAP-III (1-81) ; CXC chemokine ligand 7; CXCL 7; LA PF 4; Leukocyte derived growth factor; Low-affinity platelet factor IV; NAP 2; NAP-2 (1-63) ; NAP-2 (1-66) ; NAP-2 (73) ; NAP-2 (74) ; Neutrophil activating peptide 2; Neutrophil-activating peptide 2 (1-63) ; Pro platelet basic protein; Pro platelet basic protein (chemokine (C-X-C motif) ligand 7) ; Pro-Platelet basic protein; Small inducible cytokine subfamily B member 7; Small-inducible cytokine B7; Thrombocidin 1; Thrombocidin 2; Thromboglobulin; βTG; PBP; CXCL7; NAP2; β-thromboglobulin

UniProt

P02775

Accession Number

P02775

Expression System

E.coli

Tag

N-Sumo

Sequence

Ala59-Asp128

Field of Research

Immunology; Cardiovascular; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.59 kDa

Observed Molecular Weight

25 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human