Recombinant Human IL-15 Protein (Sumo Tag)

Specifications

Catalog number

PDEH100594-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by the IL-15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart sμggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x (L), possibly throμgh the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.

Abbreviation

IL-15

Synonyms

IL-15; Interleukin 15; Interleukin-15; IL15

UniProt

P40933

Accession Number

P40933

Expression System

E.coli

Tag

N-Sumo

Sequence

Asn49-Ser162

Field of Research

Immunology; Cancer; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.4 kDa

Observed Molecular Weight

32 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human