Recombinant Human TNF-α Protein (Gst Tag)

Specifications

Catalog number

PDEH100596-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1, CD120a, p55/60) and TNF-R2 (TNF receptor type 2, CD120b, p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Abbreviation

TNF-α

Synonyms

Tumor Necrosis Factor Ligand Superfamily Member; TNFSF; TNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha; Cachectin; Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; APC1; APC1 protein; DIF、TNF-alpha、TNFA、TNFSF2; Differentiation inducing factor; Macrophage cytotoxic factor; macrophage derived; member 2; Membrane Form; monocyte derived; soluble form; sTNFA; TNF superfamily; Tumor necrosis factor alpha

UniProt

P01375

Accession Number

P01375

Expression System

E.coli

Tag

N-Gst

Sequence

Val77-Leu233

Field of Research

Immunology; Signal Transduction; Cancer; Cardiovascular; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43 kDa

Observed Molecular Weight

45 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human