Recombinant Human CCL14a/HCC-1 Protein (Sumo Tag)

Specifications

Catalog number

PDEH100598-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CCL14a/HCC-1 has an important biological role in other mammals by evolving under positive selection that has been lost in Ochotonidae (subgenera Pika and Lagotona) . CC chemokine ligand 14, CCL14a/HCC-1, is a human CC chemokine that is of recent interest because of its natural ability, upon proteolytic processing of the first eight NH2-terminal residues, to bind to and signal throμgh the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5) . Embryo implantation is a complex process involving blastocyst attachment to the endometrial epithelium and subsequent trophoblast invasion of the decidua. We have previously shown that the chemokines CX3CL1 and CCL14a/HCC-1 are abundant in endometrial vasculature, epithelial, and decidual cells at this time, and that their receptors, CX3CR1 and CCR1, are present on invading human trophoblasts. CX3CL1 and CCL14a/HCC-1 promote trophoblast migration.

Abbreviation

CCL14a/HCC-1

Synonyms

CCL; HCC-1/HCC; SCYA; Chemokine CC-1/CC; C-C motif chemokine 14; CC-1; CC-3; Chemokine CC-1/CC-3; HCC-1; HCC-1 (1-74) ; HCC-1/HCC-3; HCC-3; NCC-2; Small-inducible cytokine A14; NCC2; SCYA14; CCL14; CKb1; MCIF; SCYL2

UniProt

Q16627

Accession Number

Q16627

Expression System

E.coli

Tag

N-Sumo

Sequence

Gly28-Asn93

Field of Research

Immunology; Signal Transduction; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20 kDa

Observed Molecular Weight

25 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human