Recombinant Human Betacellulin/BTC Protein (Sumo Tag)

Specifications

Catalog number

PDEH100599-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Betacellulin (Betacellulin/BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4) . Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. Betacellulin/BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices, 3 residues) 36% beta sheet (5 strands, 18 residues) . Betacellulin/BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Abbreviation

Betacellulin/BTC

Synonyms

BTC

UniProt

P35070

Accession Number

P35070

Expression System

E.coli

Tag

N-Sumo

Sequence

Asp32-Tyr111

Field of Research

Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.7 kDa

Observed Molecular Weight

32 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human