Recombinant Human CXCL7/NAP-2 Protein (Trx Tag)

Specifications

Catalog number

PDEH100601-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pro-platelet basic protein (PPBP) is also known as Chemokine (C-X-C motif) ligand 7 (CXCL7) and nucleosome assembly protein (Nap-2) . Nap-2 / PPBP / CXCL7 is released in large amounts from platelets following their activation and is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. Nap-2 / PPBP / CXCL7 has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Nap-2 is a ligand for CXCR1 and CXCR2, and Nap-2, Nap-2 (73), Nap-2 (74), Nap-2 (1-66), and most potent Nap-2 (1-63) are chemoattractants and activators for neutrophils.

Abbreviation

CXCL7/NAP-2

Synonyms

B-TG; CTAP; CXCL; SCYB; C-X-C Motif Chemokine; MDGFSmall-Inducible Cytokine B; LA-PF; NAP; PPBP; B-TG1; Beta-TG; CTAP-III; CTAP3; CTAPIII; CXCL7; LA-PF4; LDGF; MDGF; NAP-2; PBP; SCYB7; TC1; TC2; TGB; TGB1; THBGB; THBGB1; C-X-C Motif Chemokine 7; Leukocyte-Derived Growth Factor; Macrophage-Derived Growth Factor; MDGFSmall-Inducible Cytokine B7; NAP2; Platelet Basic Protein; βTG; B TG1; Beta TG; Beta thromboglobulin; beta-1; Chemokine (C X C motif) ligand 7; Connective tissue activating peptide III; CTAP 3; CTAP III; CTAP-III (1-81) ; CXC chemokine ligand 7; CXCL 7; LA PF 4; Leukocyte derived growth factor; Low-affinity platelet factor IV; NAP 2; NAP-2 (1-63) ; NAP-2 (1-66) ; NAP-2 (73) ; NAP-2 (74) ; Neutrophil activating peptide 2; Neutrophil-activating peptide 2 (1-63) ; Pro platelet basic protein; Pro platelet basic protein (chemokine (C-X-C motif) ligand 7) ; Pro-Platelet basic protein; Small inducible cytokine subfamily B member 7; Small-inducible cytokine B7; Thrombocidin 1; Thrombocidin 2; Thromboglobulin; βTG; PBP; CXCL7; NAP2; β-thromboglobulin

UniProt

P02775

Accession Number

P02775

Expression System

E.coli

Tag

N-Trx

Sequence

Ala59-Asp128

Field of Research

Immunology; Cardiovascular; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.6 kDa

Observed Molecular Weight

30 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human