Recombinant Human CCL15/MIP-1δ Protein (Trx Tag)

Specifications

Catalog number

PDEH100617-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 15 (CCL15/MIP-1δ), also known as leukotactin-1, MIP5, MIP1 and HCC-2, is a small cytokine belonging to the C-C chemokine family. CCL15/MIP-1δ is prevantly expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3.

Abbreviation

CCL15/MIP-1δ

Synonyms

CCL; HCC; LKN; Leukotactin; Chemokine CC; SCYA; C-C motif chemokine; Small-inducible cytokine A; Macrophage inflammatory protein; C-C motif chemokine 15; Chemokine CC-2; HCC-2; Leukotactin-1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP-5; Mrp-2b; NCC-3; Small-inducible cytokine A15; MIP5; NCC3; SCYA15; CCL15; HMRP-2B; MIP-1d; SCYL3

UniProt

Q16663

Accession Number

Q16663

Expression System

E.coli

Tag

N-Trx

Sequence

Gln22-Iie113

Field of Research

Immunology; Cardiovascular; Kits; Lysates; Other

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30 kDa

Observed Molecular Weight

31 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human