Recombinant Human CCL16/HCC-4 Protein (Trx Tag)

Specifications

Catalog number

PDEH100618-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CCL16/HCC-4, a chemokine poorly characterized at the functional level. Human CCL16/HCC-4 is a member of the CC family, and its gene maps to human chromosome 17q. In the mouse, only a pseudogene has been identified to date. CCL16/HCC-4 is a functional ligand for CCR1, CCR2, CCR5, and CCR8. Recombinant CCL16/HCC-4 demonstrated chemotactic activity on human monocytes and lymphocytes. Based on the ability of human chemokines to exert activity on and bind to murine receptors, the TSA mouse adenocarcinoma cell line was transfected with human CCL16/HCC-4 cDNA and, in comparison with other cytokines, was shown to be the faster inducer of systemic immune response due to massive, prompt infiltration of leukocytes.

Abbreviation

CCL16/HCC-4

Synonyms

HCC; Mtn; CKb; CCL; SCYA; SCYL; Monotactin; Chemokine CC; Small-Inducible Cytokine A; NCC; LCC; CCL16; CKb12; HCC-4; ILINCK; LCC-1; LEC; LMC; Mtn-1; NCC-4; NCC4; SCYA16; SCYL4; C-C motif chemokine 16; Chemokine CC-4; Chemokine LEC; IL-10-Inducible Chemokine; LCC1; Liver expressed chemokine; Liver-Expressed Chemokine; Lymphocyte and Monocyte Chemoattractant; Monotactin-1; Small-Inducible Cytokine A16; CKb12; HCC-4; LCC-1; LEC; LMC; Mtn-1; NCC-4; SCYL4; Chemokine (C-C motif) ligand 16; HCC4; IL10 inducible chemokine; Liver CC chemokine 1 precursor; Monotactin 1; Mtn1; New CC chemokine 4; Small inducible cytokine A16 precursor; Small inducible cytokine subfamily A (Cys Cys) member 16

UniProt

O15467

Accession Number

O15467

Expression System

E.coli

Tag

N-Trx

Sequence

Gln24-Gln120

Field of Research

Immunology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.5 kDa

Observed Molecular Weight

31 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human