Recombinant Human HK2 Protein (Trx Tag)

Specifications

Catalog number

PDEH100629-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of the glycolysis pathway, expresses at a high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic processes. The glycolytic enzyme hexokinase 2 (HK2) is crucial for the Warburg effect in human glioma, the most common malignant brain tumor. Althoμgh absent in most adult tissues, hexokinase 2 (HK2) is expressed in a majority of tumors and contributes to increased glucose consumption and to in vivo tumor 18F-FDG PET signaling. Hexokinase 2 (HK2) is a rate-determining enzyme in aerobic glycolysis, a process upregulated in tumor cells. HK2 expression is controlled by various transcription factors and epigenetic alterations and is heterogeneous in hepatocellular carcinomas (HCCs), thoμgh the cause of this heterogeneity is not known.

Abbreviation

HK2

Synonyms

DKFZp686M; HXK; HK2; HKII; HXK2; hexokinase-2; DKFZp686M1669; Hexokinase 2; Hexokinase 2 muscle; Hexokinase type II; HK 2; HK II; HxK 2; Muscle form hexokinase

UniProt

P52789

Accession Number

P52789

Expression System

E.coli

Tag

N-Trx

Sequence

His568-Arg917

Field of Research

Signal Transduction; Cardiovascular; Cancer; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

58.3 kDa

Observed Molecular Weight

38 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human