Recombinant Human LC3A/B Protein (Trx Tag)

Specifications

Catalog number

PDEH100633-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

LC3A, also known as MAP1LC3A, is one of the light chain subunits that function together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver, and skeletal muscle but are absent in thymus and peripheral blood leukocytes.

Abbreviation

LC3A/B

Synonyms

MAP1A/1BLC; MAP1 light chain 3-like protein; MAP1ALC; MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3A/LC3B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1ALC3; Autophagy related protein LC3 A; Autophagy related ubiquitin like modifier LC3 A; Autophagy related ubiquitin like modifier LC3 B; LC3; LC3II; MAP; MAP1 light chain 3 like protein 1; MAP1 light chain 3 like protein 2; MAP1A/1B light chain 3 A; MAP1LC3B a; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B

UniProt

Q9GZQ8

Accession Number

Q9GZQ8

Expression System

E.coli

Tag

N-Trx

Sequence

His27-Val125

Field of Research

Signal Transduction; Neuroscience; Cardiovascular; Cancer; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.7 kDa

Observed Molecular Weight

31 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human