Recombinant Mouse RETN Protein (Trx Tag)

Specifications

Catalog number

PDEM100129-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance. Mature Mouse Resistin is a 12 kDa protein with an N-terminal alpha-helical domain and a C-terminal beta-sandwich domain that is stabilized by multiple intrachain disulfide bonds. Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with an the trimeric form showing greater bioactivity. Resistin can also form multimers with an RELM beta. Mature Mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with an Human and rat Resistin, respectively. It shares 34%-42% aa sequence identity with an Mouse RELM alpha, beta, and gamma. In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus. It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet. This is in contrast to Human Resistin which is produced by macrophages and monocytes but not by adipocytes. Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance. Human Resistin promotes lipolysis by Human and Mouse adipocytes, but Mouse Resistin does not promote lipolysis by adipocytes of either species. Both Mouse and Human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo.

Abbreviation

RETN

Synonyms

Adipose tissue specific secretory factor; Adipose tissue-specific secretory factor; ADSF; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; CG5403; Cysteine rich secreted protein A12 alpha like 2; Cysteine rich secreted protein FIZZ3; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; dri; FIZZ 3; FIZZ3; Found in inflammatory zone 3; HXCP 1; HXCP1; MGC126603; MGC126609; PRO1199; Resistin; Resistin delta2; RETN 1; RETN; RETN; RETN1; RSTN; UNQ407; XCP 1; XCP1

UniProt

Q99P87

Accession Number

Q99P87

Expression System

E.coli

Tag

N-Trx

Sequence

Ser21-Ser114

Field of Research

Cardiovascular; Metabolism; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.2 kDa

Observed Molecular Weight

33 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse