Recombinant Mouse SH3Y1 Protein (Sumo Tag)

Specifications

Catalog number

PDEM100137-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Differential screening of mouse skin cDNA libraries identified cDNA encoding a protein containing a Src homologous 3 (SH3) domain, a novel protein containing a SH3 domain at the carboxyl terminus, and reporting gene expression patterns during the hair growth cycle. The protein shows significant homology with an the yeast protein YHRO 16c/Ysc 84 in its amino and carboxyl terminus, suggesting that it may be a mouse homolog of the yeast protein. Therefore, we name it the Sh3 domain YSC-like 1, Sh3yl1.

Abbreviation

Sh3yl1

UniProt

O08641

Accession Number

O08641

Expression System

E.coli

Tag

N-Sumo

Sequence

Met1-Asn340

Field of Research

Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.3 kDa

Observed Molecular Weight

55 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse