Recombinant Mouse IL-27 Protein (Sumo Tag)

Specifications

Catalog number

PDEM100180-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells, and dendritic cells. IL-27 protein is also referred to as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), and IL-12 p40-related protein (IL-27B) . IL-27 protein is an early product of activated antigen-presenting cells and drives the rapid clonal expansion of naive CD4 (+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas throμgh WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.

Abbreviation

IL-27

Synonyms

IL-T; Il27a; Il27; IL-27; IL-27A; IL27p28; IL30; MGC71873; p28

UniProt

Q8K3I6

Accession Number

Q8K3I6

Expression System

E.coli

Tag

N-Sumo

Sequence

Phe29-Ser234

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.5 kDa

Observed Molecular Weight

42 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse